Oligomycin-induced energization of submitochondrial particles
نویسندگان
چکیده
منابع مشابه
Oligomycin-sensitive ATPase of Submitochondrial Particles from Corn.
To test the hypothesis (Plant Physiology 59: 155-157) that monocotyledons contain a unique oligomycin-insensitive ATPase, we prepared submitochondrial particles and a soluble fraction from sonicated corn mitochondria (Zea mays L. cv. Earliking). Although the ATPase activity of the whole sonicate was relatively insensitive to oligomycin, the corn submitochondrial particles possessed an ATPase ac...
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Incorporation of the fluorescent, nonpermeant pH indicator pyranine into submitochondrial particles (pyranine-SMP) permitted monitoring of intravesicular pH changes brought about by proton translocation due to oxidation of respiratory chain substrates or to hydrolysis of ATP. Addition of oligomycin to beef heart pyranine-SMP was followed by a pH-independent quenching of pyranine fluorescence. Q...
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1. The maximal H+: 0 ratios of proton transport in highly resolved submitochondrial particles prepared by sonication of bovine heart mitochondria in the presence of ammonia and exposed sequentially to Sephadex and urea (ASU particles) in the presence of oligomycin were found to be 6,4, and 2 during the oxidation of NADH, succinate, or N-methylphenazonium methosulfate, respectively. A pH depende...
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1. Ca++ accumulation was shown to take place at pH 7.0 in submitochondrial particles. The particles were prepared from bovine heart mitochondria either by digitonin treatment or by sonic oscillation followed by osmotic shock and sedimentation at 26,000 X g. Both succinate and ATP (or ADP) were required for Ca++ accumulation. Carbonylcyanide ptrifluoromethoxyphenylhydrazone abolished the process...
متن کاملInteraction of fluorescent probes with submitochondrial particles during oxidative phosphorylation.
Addition of several N-arylaminonaphthalenesulfonate dyes to submitochondrial particles from bovine heart resulted in an enhancement of fluorescence of from SOto 150-fold and a considerable shift in the wave length of maximum emission toward the ultraviolet. The results indicate 40 to 55 dyebinding sites per mg of submitochondrial particles. A preliminary search revealed that mitochondrial ATPas...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1160013p